Solution structure of the Shc SH2domain complexed …
Proc. Natl. Acad. Sci. USA92 (1995) 7785 15N-, 13C-labeled protein (2 mM) and unlabeled phos- phopeptide (4 mM)in a 2H20 or a H20/2H20 (9:1) buffer (pH6.5) containing50mMTris-dll, 50mMNaCl,and5 mM dithiothreitol-dio. NMRSpectroscopy. NMRexperimentswereperformedat 20°ConaBruker(Billerica, MA)AMX500orAMX600NMR